Biothiol-Triggered Hydrogen Sulfide (H2S) Release from Near-Infrared Fluorescence H2S Donor for Promoting Cutaneous Wound Healing

Preprint | 
10.55415/deep-2022-0055.v1
This is not the most recent version. There is anewer versionof this content available.
Xinyue Zhao#
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China
Mengbin Ding#
Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
Lulu Ning
Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi’an 710021, P. R. China
Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi’an 710021, P. R. China
Fang Yuan
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China
Jingchao Li
Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
Yuan Guo
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China
Yuguang Mu
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
Jianjian Zhang*
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China++Qinghai Provincial Key Laboratory of Salt Lake Resources Chemistry, Xining, 810008, P. R. China
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi 710127, P. R. China++Qinghai Provincial Key Laboratory of Salt Lake Resources Chemistry, Xining, 810008, P. R. China

# contributed equally to this work, * Corresponding author

Abstract

Hydrogen sulfide (H2S) plays crucial roles in antioxidation, anti-inflammation, and cytoprotection. Despite the great progress in the design and synthesis of activatable H2S donors, high-precision detection and imaging of released H2S in living systems is still an outstanding issue. In this study, a biothiol-activated near-infrared (NIR) fluorescent H2S donor PRO-ST for real-time visualization of H2S release is developed. PRO-ST, consisting of a dicyanoisophorone-based NIR fluorescence moiety (TCOO), a biothiol-trigger moiety (4-isothiocyanate benzyl alcohol), and a sulfur-source group (thiophosgene), is characterized by large NIR fluorescence enhancement (45-fold), outstanding H2S release efficiency (73%), prominent controllable H2S release (60 min) and excellent cell compatibility. The distinctive features render PRO-ST capable of visualizing H2S release in cells, zebrafish, and mice. Moreover, PRO-ST has exhibited excellent performance of real-time anti-inflammation and wound healing enhancement in biological systems, which has been confirmed by in-situ visualization of H2S release.  The versatile PRO-ST provides possible and effective solution to the detection and visualization of H2S release, which will benefit the comprehension of underlying mechanisms of wound healing and the optimization of interventional therapy.

Download PDF
CITE
Keywords
H2S donor ;  Biothiol-activated ;  Anti-inflammatory ;  Wound healing
Subject Area
Cell Biology
Now Published
Version History
  • 16 Nov 2022 12:11 Version 1
Scores
 4
Rapid Rating Times: 1
· Level of Quality: 4
· Level of Repeatability: 4
· Level of Innovation: 5
· Level of Impact: 3

*Each rating ranges from 0-5

Rapid Rating
Your professional field is different from the direction of this article. Go Settings!
  • Level of Quality
    Is the publication of relevance for the academic community and does it provide important insights? Is the language correct and easy to understand for an academic in the field? Are the figures well displayed and captions properly described? Is the article systematically and logically organized?
    0.0
  • Level of Repeatability
    Is the hypothesis clearly formulated? Is the argumentation stringent? Are the data sound, well-controlled and statistically significant? Is the interpretation balanced and supported by the data? Are appropriate and state-of-the-art methods used?
    0.0
  • Level of Innovation
    Does the work represent a novel approach or new findings in comparison with other publications in the field?
    0.0
  • Level of Impact
    Does the work have potential huge impact to the related research area?
    0.0
Submit
Not yet Peer Reviewed
The content is available under CC BY 4.0 License CreativeCommons.org
The author(s) have declared they have no conflict of interest with regard to this content

我们使用 cookie 将您与其他用户区分开来, 并在我们的网站上为您提供更好的体验。

关闭此消息以接受 cookie 或了解如何管理您的 cookie 设置。

了解更多关于我们的隐私声明..

goTop